Figure 6.

Effect of FASN over-expression on gemcitabine response. A. FASN expression level. Lysates were prepared from Panc-1 cells with stable trans-fection with FASN cDNA (FASN3 and FASN7) or vector (Vec) control and used for Western blot analysis of FASN and actin loading control. B. Survival assay. Panc-1 stable clones were treated with various concentrations of gemcitabine followed by analysis using MTT assy. C. Relative resistance factor. Relative resistance factor (RRF) to gemcitabine was derived as described in Materials and Methods. ** p<0.01.