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SMART compounds inhibit tubulin polymerization via binding to the colchicine binding site on tubulin. (A) Structures of SMART-H, -F, and –OH. (B) Effect of SMART compounds on tubulin polymerization. Tubulin (0.4 mg) was exposed to SMART compounds (10 μM). Absorbance at 340 nm was monitored every min for 15 min.
