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. 2010 Dec 23;9(1):1–28. doi: 10.3390/md9010001

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© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Growth of the Mn(II)-oxidizing bacterium strain BAC-SubDo-03 isolated from S. domuncula. Extracts from the sponge were prepared and plated on Mn agar. Colonies of Mn(II)-oxidizing microorganisms were picked and subsequently cultivated (7 d) in liquid K-medium. This medium either lacked Mn (A), or was supplemented with Mn (100 μM MnCl₂) (B). (C) Cultivation (for 72 hrs) of strain BAC-SubDo-03 on agar, lacking Mn or (D) containing 100 μM MnCO₃. (E) At higher magnification it is apparent that on agar medium without Mn the colonies were whitish, whereas on agar supplemented with Mn the colonies exhibited a brownish area around the colonies indicating Mn deposition (F).