Figure 10.

Expression of mnxG gene in BAC-SubDo-03, grown, in absence of Mn (minus), or presence of Mn (plus). As described under “Experimental Section”, and schematically outlined in Figure 2C, the cultures were incubated for 4 hrs, 16 hrs, and 32 hrs (lanes 1 to 3) in the absence of Mn; or, the microorganisms were cultivated in the absence of Mn and then exposed for 0.5 hr, 1 hr, 2 hr or 18 hrs to 100 μm MnCl₂ (lanes 4 to 7). The same quantity of bacteria was taken after the indicated period of incubation. Then, RNA was extracted and the level of transcripts was semi-quantitatively determined by RT-PCR. The molecular size markers (M) are shown in lane 8. In parallel, amplifications by RT-PCR in an assay supplemented with the mnxG-SubDo-03 containing plasmid (positive control [pc]), or with an empty pGEM-T vector (negative control [nc]). This PCR gel analysis is representative for five separate analyses performed.