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. 2010 Dec 23;9(1):1–28. doi: 10.3390/md9010001

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© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Growth kinetics and spore formation of strain BAC-SubDo-03 in K-medium (pH 6.8) in absence (A) or presence (B) of 100 μM MnCl₂. Bacterial growth was measured spectrophotometrically as an increase of optical density at 600 nm [bars] (mean values and standard deviations [SD] from five independent experiments are given). The percentage of spores (sporangia, free spores) was determined microscopically after staining of the samples with malachite green/safranin; these values are given as dots connected by a line. (C) Scheme representing the time points at which bacteria were harvested to determine the expression level of the mnxG gene by polymerase chain reaction (PCR) in the Bacillus strain SubDo-03 in absence (minus Mn) or presence of 100 μM MnCl₂ (plus Mn). The data of the respective RT-PCR reactions are given in Figure 9.