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. 2010 Dec 23;9(1):1–28. doi: 10.3390/md9010001

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© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Capacity of the sponge associated BAC-SubDo-03 bacteria to oxidize Mn(II) in the medium. The bacterial cultures (+ bacteria; darker shaded columns) were incubated in K medium with increasing concentrations of MnCl₂ (50 μM to 200 μM) for 6, 12 or 24 hrs, as indicated. In parallel, medium was supplemented with MnCl₂ for the same period of time. As a control, uninoculated medium (-bacteria; brighter shaded bars) with added MnCl₂ was run in parallel with the inoculated medium. Five independent experiments were performed and the mean values (means and SD) are shown; significance: *P < 0.001. Aliquots (2 mL) from the cultures were assayed for the concentration of the oxidized manganese in the medium. The amount of oxidized Mn(II) was determined by the colorimetric dye leucoberberlin blue which reacts with Mn(III) and Mn(IV). The respective optical densities were converted to μM of Mn(IV) by using K-permanganate as reference material.