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Binding of manganese to BAC-SubDo-03 bacteria. The microorganisms were incubated in K medium, supplemented with radiolabeled manganese, as described under “Experimental Section”. Then aliquots were taken and the activity was determined. The total amount of manganese immobilized by the bacteria is given in nmol/mg of bacterial protein. The mean values, together with SD, from five independent experiments are given; significance: *P < 0.001.
