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. 2010 Dec 23;9(1):1–28. doi: 10.3390/md9010001

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© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Sequence relationship of the mnxG gene (termed mnxG-SubDo-03), identified in the Bacillus-related strain, isolated from S. domuncula. (A) Schematic representation of the mnx region with the central gene MnxG-SubDo-03 attributed to the MCO, taken from Dick et al. [11]. The locations of the putative Cu-binding regions within MnxG-SubDo-03, the regions E to F, have been indicated. The primers to identify the MCO from the sponge-associated bacteria (BAC-SubDo-03) span the regions C to B; the respective locations are indicated using the numbering of the gene isolated from the bacterial strain SG-1 [12]. The consensus Cu-binding sequence motifs (C/D and A/B) found in all MCOs are given. (B) The polypeptide, MnxG-SubDo-03, deduced from the partial nucleotide sequence (mnxG-SubDo-03) was compared with the MnxG proteins from the following Bacillus strains [11–13]: strain PL-12 (MnxG-PL-12; ABP68890.1), strain GB02-31 (MnxG-GB02-31; AAZ31744.1), strain GB02-30 (MnxG-GB02-30; AAZ31743.1), strain GB02-27 (MnxG-GB02-27; AAZ31742.1), strain GB02-25 (MnxG-GB02-25; AAZ31741.1), strain GB02-16 (MnxG-GB02-16; AAZ31739.1), strain GB02-14C (MnxG-GB02-14C; AAZ31738.1), strain GB02-8B (MnxG-GB02-8B; AAZ31736.1), strain SD-18 (MnxG-SD-18; AAL30449.1), strain GB02-8B (MnxG-GB02-8B; AAZ31736), strain GB02-18 (MnxG-GB02-18; AAL30449.1) and strain GB02-2A (MnxG-GB02-2A; AAZ31735.1). These sequences were aligned, the tree computed and rooted with the sequence from Bacillus strain MB-7 (ABP68890) [see Figure 3]. The bootstrap values base on 1,000 replicates and are indicated at the branch points. The grouping of the sequences was performed as outlined by Dick et al. [11] and in Figure 3.