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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Prostate. 2010 Nov 1;70(15):1658–1671. doi: 10.1002/pros.21201

Fig. 5.

Fig. 5

In vitro suppression or loss of Pten is not sufficient to antagonize 1,25(OH)2D3-mediated growth suppression. A: Growth inhibition of WFU3MPECs infected with Pten shRNA or scrambled shRNA in response to 1,25(OH)2D3. Values are means for thetriplicates ± SD. B: Pten and phospho-Akt levels of WFU3MPEC clones infected with lentivirus expressing scrambled shRNA (control clones) or shRNA targeting Pten (Pten shRNA clones) as determined by Immunoblot. C: Growth inhibition of Ptenlox/lox and Pten−/− MPECs in response to 1,25(OH)2D3. Ptenlox/lox MPECs isolated from prostates of Ptenlox/lox Cre-recombinase negative animals and infected with lentivirus expressing self-deleting Cre-recombinase to generate Pten−/− MPEC. Values are means for the triplicates ±SD. D: Pten and phospho-Akt levels of Ptenlox/lox and Pten−/− MPECs determined by Immunoblot. Protein lysate isolated from LNCaP cells was used as controls.