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Proliferation and metabolic activity of C2C12 cells stimulated with indirect LIUS. (a) C2C12 cells were stimulated for 3 or 10 minutes daily for 6 days and total cell number was measured by trypan blue exclusion methods on day 6. The control was not stimulated (P < .05, N = 8). (b) Lactate concentration in the culture media was measured at days 2, 4, and 6 (N = 4). LIUS stimulation increased cell proliferation and metabolic activity of C2C12 cells.
