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. 2011 Feb;3(2):a004648. doi: 10.1101/cshperspect.a004648

Figure 2.

Figure 2.

Tube pulling and fluorescence images. (A) A nanoscale-radius tube is extracted from a micron-scale GUV that is in turn held by aspiration in a micropipette (left side of image). The tube is held by a Polystyrene bead in an optical trap (indicated by white circle). Here, the membrane composition is far from phase separation. Confocal fluorescence shows that the dye compositions in the tube and GUV are the same. (B) The membrane composition is close to phase separation (BSM:Chol:DOPC 30:35:35 mole%). Quantitative fluorescence measurement shows a 2.9-fold relative enrichment of the red (Texas red DHPE) over green (BodipyFL-C5-GM1) in the tube relative to the GUV. Scale bar, 5 µm.