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. 2011 Feb 3;12:6. doi: 10.1186/1471-2091-12-6

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Copyright ©2011 Yamakoshi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dentin power extraction diagram. The extracts, in bold, are the first guanidine extract (G1), first guanidine pellet (G1P), acid (A), acid salt (AN), Tris salt (TN), residual insoluble material (RIM), second guanidine extract (G2), and the second guanidine pellet (G2P). Selected extractions were fractionated to isolate Dspp cleavage products. The AN extract was fractionated by size exclusion (S) and RP-HPLC (R) to yield HMW Dsp in fractions ANS1/2-R3, ANS1/2-R4, and ANS1/2-R5. The A extract was fractionated by size exclusion and RP-HPLC to yield LMW Dsp (AS2R-g). Chondroitinase activity was detected in the second ion exchange (Q) fraction of the G2 extact (G2Q2).