Figure 5.

Characterization of glycosylated pronase digestion products of ANS1/2-R4. A: Size exclusion chromatogram (Abs²²⁰ v. min) of ANS1/2-R4 digested with pronase (top); tubes showing results of phenol sulfuric acid assay performed on each size exclusion fraction (bottom; B is "blank" for negative control). B: RP-HPLC chromatogram (Abs²²⁰ v. min) of digestion products in ANS1/2R4-Pr1 showing the 8 fractions (a-h) collected. C: N-terminal sequences determined for the contents of each fraction peak (Pk). Blank cycles (x) indicate modified residues.