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. 2011 Jan 28;5:1. doi: 10.1186/1754-1611-5-1

Figure 6.

Figure 6

LVs produced with DMJ transduced DCSIGN-expressing cells much more efficiently than LVs produced without DMJ. (A) LVs were produced either with or without DMJ and spin-infected with 293T and 293T.DCSIGN cells. Although FUGW/SVGmu(DMJ-) preferentially transduced 293T.DCSIGN cells, FUGW/SVGmu(DMJ+) was over three-fold more efficient in transducing 293T.DCSIGN cells while maintaining similar levels of background transduction to 293T cells. (B) LVs produced with DMJ yielded higher vector titers for DCSIGN-expressing cells compared with LVs produced without DMJ. Vector titers for both FUGW/SVGmu +/- DMJ were analyzed for 293T.DCSIGN and 293T cells. On 293T.DCSIGN cells, FUGW/SVGmu(DMJ+) yielded titers over three-folds higher than those of FUGW/SVGmu(DMJ-), while titers for 293T cells for both vectors were similarly low. FUGW/VSVG +/- DMJ was included as a control.