Table 4.
Differentially expressed proteins in ΔsRNA-Xoo1 identified by MS
| Spot ID | protein name | functions | NCBI acc.no. | mascot score | Sequence coverage | theoretical MW(Da)/pI |
Ratio ± SD |
|---|---|---|---|---|---|---|---|
| Down-regulated protein spots in sRNA-Xoo1 mutant | |||||||
| B1 | putative ABC transporter ATP-binding protein | transport | PXO_04409 | 448 | 56% | 61740/5.37 | 11.57 ± 4.83 |
| B2 | glyceraldehyde-3-phosphate dehydrogenase | metabolism | PXO_02308 | 529 | 69% | 36134/6.35 | 4.75 ± 0.57** |
| B3 | argininosuccinate synthase | amino acid biosynthesis | PXO_00352 | 769 | 67% | 45217/5.66 | 3.25 ± 0.56 |
| B4 | tryptophan repressor binding protein | amino acid metabolism | PXO_03044 | 438 | 52% | 20167/6.05 | 2.83 ± 0.18** |
| B5 | 3-isopropylmalate isomerase large subunit | amino acid biosynthesis | PXO_02613 | 314 | 42% | 51984/5.68 | 1.86 ± 0.21** |
| B6 | N-acetylornithine carbamoyltransferase | amino acid biosynthesis | PXO_00353 | 314 | 65% | 37582/6.07 | 1.7 ± 0.19** |
| B7 | superoxide dismutase | oxidation reduction | PXO_00389 | 343 | 68% | 22703/5.47 | 1.42 ± 0.03** |
| B8 | septum site-determining protein MinD | replication | PXO_04464 | 435 | 46% | 28837/5.21 | 1.57 ± 0.19** |
| B9 | N-ethylammeline chlorohydrolase | metabolism | PXO_00380 | 263 | 62% | 48943/5.19 | ND |
| B10 | 2-isopropylmalate synthase | amino acid biosynthesis | PXO_02609 | 694 | 71% | 53421/5.54 | 1.54 ± 0.15** |
| B11 | translation elongation factor G | transcription and translation | PXO_04525 | 642 | 58% | 75924/5.09 | 4.82 ± 0.42** |
| B12 | dihydrolipoamide dehydrogenase | amino acid metabolism | PXO_01196 | 582 | 67% | 50445/5.98 | 2.29 ± 0.54 |
| B13 | N-acylglucosamine 2-epimerase | metabolism | PXO_01231 | 811 | 77% | 46884/5.27 | 2.23 ± 0.34** |
| B14 | ATP synthase subunit alpha | transport | PXO_03111 | 777 | 48% | 55391/5.38 | 2.23 ± 0.5 |
| B15 | phosphoenolpyruvate synthase | Pyruvate metabolism | PXO_00922 | 622 | 48% | 86689/5.16 | 6.36 ± 1.69 |
| B16 | Polyribonucleotide nucleotidyltransferase | metabolism | PXO_01307 | 729 | 55% | 75502/5.47 | 5.39 ± 0.93 |
| Up-regulated protein spots in ΔsRNA-Xoo1 mutant | |||||||
| B17 | pyrroline-5-carboxylate reductase | amino acid metabolism | PXO_01991 | 301 | 51% | 29364/4.93 | 1.48 ± 0.07** |
| B18 | peptide deformylase | protein synthesis | PXO_04055 | 379 | 55% | 17129/5.29 | 1.45 ± 0.02** |
| B19 | outer membrane protein | transport | PXO_03097 | 343 | 32% | 23707/5.27 | 1.43 ± 0.04** |
| B20 | Chaperone protein dnaJ | DNA replication | PXO_01186 | 702 | 64% | 41079/6.21 | 1.52 ± 0.14** |
| B21 | hydrolase, carbon-nitrogen family | metabolism | PXO_06060 | 526 | 68% | 30229/5.89 | 1.57 ± 0.12** |
| B22 | hypothetical protein | hypothetical protein | PXO_01766 | 635 | 75% | 16502/6.3 | 1.41 ± 0.01 |
ND: not detectable in the 2-DE maps of wild-type or ΔsRNA-Xoo1 mutant. SD: Standard Deviation. Statistical significance was determined using Student's two-tailed t test for unpaired means P < 0.05, P < 0.01**.