Figure 2.

FISH of 11 fosmids on cucumber somatic and pachytene chromosomes. a FISH of 11 fosmids on somatic chromosomes. Adjacent probes were labeled with different fluorochromes and hybridized together to test the order and position. b Cucumber chromosomes at the pachytene stage were probed by a set of eleven fosmid clones together with the Type III satellite repeat. c Three straightened cucumber pachytene chromosome 2. The left chromosome is straightened from the image shown in Figure 2b. d The chromosomes in Figure 2c were converted into black-white image. Distinct heterochromatin is visible at the centromeric region and at the distal ends of both chromosome arms. Bars, 5 μm