Figure 6. Phagocytosis enhanced by rCfLec-1.

The hemocytes were resuspended with TBS buffer, rTrx in TBS buffer and rCfLec-1 in TBS buffer, respectively. The hemocytes were incubated for 30 min, then E. coli was added into each hemocytes suspension for another 1 h. The mixture was mounted onto a glass slide and stained with Giemsa. The phagocytic activity of hemocyte was measured using a light microscope. Two hundred hemocytes on each slide were counted. Phagocytic rate (PR)  =  (phagocytic hemocytes)/(total hemocytes)×100%; phagocytic index (PI)  = average number of bacteria in phagocytic hemocytes. For each treatment, assay was performed in three different slides for statistic analysis.