Figure 8. IL-23 is not required for IL-22 production during a secondary systemic LM infection.

B6 and IL-23p19 KO were i.v. infected with ∼1×10³ LM, then re-infected six-weeks later with ∼1×10⁶ LM. Serum and spleens were harvested 2 days p.i. The concentration of IL-22 was measured using ELISA in serum (A) and overnight splenocyte supernatants from un-stimulated (B), HKLM stimulated (C), or IL-23 stimulated (D) cultures. A two-way ANOVA did not detect significant effects of mouse strain (p>0.05). These data are representative of two independent experiments. All data are expressed as the mean + SEM (n = 4/group).