Table 1. Cellular composition of the splenic explant culture from hamsters infected with L. donovani.

		Days post-infection 1
Cell type	Result	0	7	14	21
Splenocytes 2	Total 2	35.0±10.8	86.6±56.6	119±23.3 c	135.8±16.0 b
CD3+ T Cells	% 3	21.6±5.4	23.3±3.7	13.6±3.5 c	18.5±0.5
	No. 4	7.6±1.9	20.2±3.2 d	16.1±4.2 d	25.1±0.7 d
CD4+ T Cells	%	5.6±1.9	6.9±3.5	2.6±0.5 c	6.1±0.9
	No.	1.8±0.7	6.0±3.0 b	3.1±0.5	8.3±1.3 a
B Lymphocytes	%	14.6±2.0	20.3±5.8 c	23.7±4.6 b	18.1±1.3
	No.	5.1±0.7	17.6±5.0	28.3±5.5 a	24.6±1.8 b
Macrophages	%	15.4±4.3	17.4±3.0	9.5±2.2	23.8±2.6 c
	No.	5.4±1.5	12.0±7.1	11.4±2.6	32.3±3.5 a
Granulocytes 5	%	3.15±1.2	0.96±0.4	1.44±0.2	0.46±0.1

¹Determined in 5 uninfected hamsters and 5 infected hamsters at each time point. The value represents the mean ± standard deviation of the group.

²Number of total splenocytes (millions) obtained from the spleen of the animals. Enumerated in a Neubauer chamber by light microscopy.

³Percentage of positive cells determined by flow cytometry.

⁴Number of cells (millions): calculated from the total number of cells multiplied by the percentage of cells positive by flow cytometry.

⁵Granulocytes: percentage of granulocytes determined by Side Scatter and Forward Scatter of cells by flow cytometry.

^ap<0.001 uninfected vs. infected groups;

^bp<0.01; uninfected vs. infected groups;

^cp<0.05, uninfected vs. infected groups (Kruskal-Wallis test);

^dp<0.001, uninfected vs. infected groups (Tukey-Kramer Multiple comparisons test).