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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Anal Chem. 2010 Oct 15;82(20):8446–8455. doi: 10.1021/ac1015358

Table 1. Dye loading efficiency and oxygen sensitivity of various G2-loaded nanosensors.

nanosensor ID1 input dye-loading Efficiency2 (%) dye loading per nanosensors (%wt) sensitivity3
G2/Total monome r (μg/mg) EDC/G2 (mol/mol) APMA/G2(mol/mol) RN2/RAir RN2/RO2 QDO-Air QDO-O2
Enc-PAA 2.6 - - 0.7% 0.0018 6.8 23 85% 96%
Enc-AFP-1 2.9 - 540 24% 0.069 3.7 9.9 73% 90%
Enc-AFP-2 4.5 - 188 22% 0.099 5.4 17 82% 94%
Cov-AFP-1 2.5 92 676 62% 0.16 2.3 6.6 57% 85%
Cov-AFP-2 4.5 98 419 67% 0.30 4.7 13 79% 92%
Cov-AFP-3 4.7 18 188 64% 0.30 8.0 28 88% 96%
Cov-AFP-4 9.5 8.9 93 46% 0.44 7.4 25 87% 96%
Cov-AFP-5 16.8 18 53 72% 1.2 7.5 25 87% 96%
Cov-AFP with BSA 4.8 18 188 21% 0.10 2.9 6.6 66% 85%
1

The nanosensor ID indicates the dye loading method as well as nanoparticle matrix. Enc and Cov denote encapsulation and covalent bonding of G2 dyes inside nanoparticles, respectively, while PAA and AFP denote polyacrylamide and amine-functionalized polyacrylamide matrixes, respectively.

2

The dye loading efficiency is defined as the percentage of the loaded dye amount with respect to the input amount.

3

The sensitivity values in this table, represented by RN2/RAir (or O2) and QDO were measured at room temperature.