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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Int J Cancer. 2011 May 1;128(9):2038–2049. doi: 10.1002/ijc.25562

Figure 5. Examination of IL-8 level and detection of microvessel density in xenograft of colon cancer cells.

Figure 5

A, Examination of tumor growth in xenograft of colon cancer cells. HCT116, HCT116-E2, Caco2 and Caco2-IIIe subcutaneously injected into nude mice. The graphs indicate the mean tumor growth rates ±SD of three animals per experimental condition. P < 0.05 was obtained by ANOVA. B, Sera IL-8 productions were measured by ELISA, and the results were normalized to whole blood volume. C and D (bottom panel), Immunohistochemistry analysis of IL-8 expression and localization of microvessel in tumor tissue slides. In HCT116 and HCT116-E2 tumor specimens, rabbit anti-human IL-8 or rat anti-mouse CD31 antibodies were added to tissue sections. All sections imaged by Zeiss Axiovision software program. The images are presented at 200× magnification. D (top panel), Blood vessel density was quantified by counting the total number of CD31-positive vessels across the whole section of tumors. For these analyses, six different tumor samples were evaluated per experimental condition. Data shown represent the mean ± standard deviation (*, P<0.05; **, P<0.005).