Figure 5.

Enhanced co-localization of cleaved caspase-8 with LC3-positive punctates in cells treated with TRAIL and cathepsin inhibitors. Bax^-/- Hct116 cells were treated daily with TRAIL (A) or with TRAIL and E64D/pepstatin A (B). Cytospins that were prepared at the indicated time points were all stained and assessed by confocal microscopy at the same time. The co-accumulation of cleaved caspase-8 with LC3 is appreciably increased early after TRAIL treatment (24 hr) with a marked reduction in the following days (A); the accumulation of cleaved caspase-8 is most pronounced with less reduction in subsequent days in cells treated with TRAIL and E64D/pepstatin A (B).