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. 2010 Oct 16;6(7):891–900. doi: 10.4161/auto.6.7.13038

Figure 6.

Figure 6

Differential patterns of lysosomal accumulation of cleaved caspase-8 as compared to cleaved caspase-3 in Bax-/- Hct116 cells treated with TRAIL and cathepsin inhibitors. Bax-/- Hct116 cells were treated daily with a fresh dose of TRAIL (100 ng/ml) for three consecutive days in the absence (A and C) or presence (B and D) of E64D/pepstatin A. The staining was performed on adherent cells in chamber slides. Of note, degradation of cleaved caspase-8 (A and B), but not of caspase-3 (C and D), is inhibited by E64D/pepstatin A. Scale bars = 40 µm. (E and F) Quantitation of co-localization with LAMP2 of cleaved caspase-8 (E) and cleaved caspase-3 (F). The co-localization was measured by Metamorph (Molecular Devices, Downingtown, PA) and performed on cells obtained from at least three independent experiments. The data are means ± SE M of 20–25 individually analyzed stained cells per sample.