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Binding of CR1 (panel A) and anti-C3 mAb 7D18.1 (panel B) and of CR2 (panel C) and anti-C3 mAb 7D323.1 (panel D) to human C3 fragments bound to adsorbed human IgG. The coated surfaces were incubated first with human serum in 3-fold serial dilution from 100% at 37°C for 60 min. After washing, heat-inactivated human serum was then added, together with buffer (solid lines) or soluble CR1 to enable digestion of deposited iC3b to C3dg (dashed lines).
