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. 2010 Nov 16;104(1):155–165. doi: 10.1038/sj.bjc.6605994

Figure 5.

Figure 5

Endogenous TSPAN8 silencing has no impact on cell survival, proliferation and migration. T1C3 melanoma cells were transfected with siRNA targeting TSPAN8 (siTSPAN8) or siRNA scramble (scramble). (A) Cell viability determined by XTT assay 3 days post-siRNA treatment of T1C3 melanoma cells. Data are expressed as mean OD 450 nm of siTSPAN8-treated cells/mean OD 450 nm in scramble siRNA-treated cells, ±s.d. of three independent experiments. (B) Cell proliferation determined by BrdU assay 3 days post-siRNA treatment of T1C3 melanoma cells. Data are expressed as in A. (C) Cells grown to confluence in uncoated, collagen I- or collagen IV-coated six-well plates were wounded by creating a scratch across the monolayer culture 3 days after transfection. Representative photographs showing this region were taken directly following injury (0 h) and at various time later using a Sony DXC-390 digital camera under an inverted phase microscope (Zeiss LSM510, Zeiss Inc., Thornwood, NY, USA). (D) Quantification of wound closure depicted in C. Data are expressed as percentage of the initial wound size and set to 100% at 0 h. No statistically significant difference was observed at any time point (P>0.05).