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. 2001 Feb 1;29(3):622–628. doi: 10.1093/nar/29.3.622

Figure 2.

Figure 2

Schematic diagram of Tet-On expression system. The 569 bp of the 3′UTR containing the TFO binding site is located between the bcl-2 ORF and SV40 polyA of the response plasmid pTRE-bcl2-tri. The bcl-2 gene is under the control of the promoter PhCMV-1, which consists of TRE, and Pmin CMV. HeLa cells that have been stably transfected with regulatory plasmid pTet-On constitutively express rtTA which is a fusion of the wild-type Tet repressor (TetR) to the VP16 AD of herpes simplex virus. When HeLa cells are transfected with pTRE-bcl2-tri, rtTA binds to the TRE and activates transcription in the presence of Doxycyclin.