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Western blot analysis showing the inhibitory effect of TFO2 on the bcl-2 gene expression at differing oligonucleotide concentrations (ODN amounts: 800, 400, 100, 10, 1 pmol). Plasmid pTRE-bcl2-tri was incubated with different concentrations of specific TFO (TFO2) and SCR, and transfected into HeLa cells that have been stably transfected with plasmid pTet-On. Cells were lysed 24 h post-transfection and western blots were carried out.
