Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 7;104(1):128–137. doi: 10.1038/sj.bjc.6606013

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2011 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Leptin induces the expression of IL-1 system in 4T1 cells. (A) Representative results of leptin-induced increase in protein levels of IL-1 system as determined by immunocytochemistry (magnification × 40). Control cells in basal conditions: (Aa) no antibodies; (Ac) IL-1α antibodies; (Ae) IL-1β antibodies; (Ag) IL-1R tI antibodies. Cells incubated with leptin: (Ab) no antibodies; (Ad): IL-1α antibodies; (Af) IL-1β antibodies and (Ah) IL-1R tI antibodies. Protein levels of IL-1 ligands (B, IL-1α; C, IL-1β) and antagonist (D, IL-1Ra) as determined by ELISA (pg ml⁻¹ per mg protein). (E), Protein levels of IL-1 ligands and receptor as determined by western blot (WB). (F) WB results were normalised to β-actin as a control and densitometric analysis of bands was carried out with the imageJ software. mRNA levels of IL-1 ligands (G, IL-1α; I, IL-1β), antagonist (H, IL-1Ra) and receptor (J, IL-1R tI) as determined by real-time RT–PCR. GAPDH was used as internal control. 4T1 cells were cultured for 24 h and leptin dose-induced (0, 0.6, 1.2 and 6.2 nM) effects were determined as described (see Materials and Methods). (a) P<0.05 when comparing levels of protein or mRNA to control (basal). Data (mean±s.e.) are representative of the results derived from a minimum of three independent experiments.