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. 2010 Dec 7;104(1):128–137. doi: 10.1038/sj.bjc.6606013

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Copyright © 2011 Cancer Research UK

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Leptin-induced signalling pathways involved in the regulation of IL-1 system in 4T1 cells. Effects of leptin and kinase inhibitors on levels of IL-1α (A), IL-1β (B), IL-1Ra (C) and IL-1R tI (D) as determined by ELISA and western blot, respectively. 4T1 cells were treated with leptin (0 or 1.2 nM) for 24 h in the presence of inhibitors of JAK2/STAT3 (AG490, 30 μM), MEK/MAPK/ERK1/2 (PD98059, 30 μM), PI-3K/AKT1 (wortmannin, 20 μM), PKC-Ca dependant (Gö6976, 30 μM), p38 kinase (SB203580, 30 μM), JNK (SP600125, 30 μM) and mTOR (Rapamycin, 20 μM) signalling pathways. WB results were normalised to β-actin as a control and densitometric analysis of bands was carried out with the imageJ software. (a) P<0.05 and (b) P<0.01 when comparing levels of protein to control with or without inhibitors (basal), respectively. Data (mean±s.e.) are representative of the results derived from a minimum of three independent experiments.