Table 2. Breakdown of studied cases by intensity of expression of ANXA6, HSP27, PRDX2, NCF2, and TPM4 (A) and histoscore (B).
| (A) | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Breakdown of cases by the intensity of expression of protein, number of patients (%)
|
|||||||||||||||||||
|
ANXA6, cytoplasmic expression
|
ANXA6, sporadic cells
|
HSP27, cytoplasmic expression
|
HSP27, nuclear expression
|
HSP27, stroma
|
|||||||||||||||
| Diagnosis | 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ |
| SCE | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 (100.0) | 0 | 0 | 0 | 0 | 0 | 5 (62.5) | 3 (36.5) | 0 |
| CIN2 | 3 (42.8) | 2 (28.6) | 2 (28.6) | 0 | 1 (25) | 0 | 0 | 3 (75.0) | 0 | 0 | 1 (25.0) | 3 (75.0) | 2 (50.0) | 1 (25.0) | 1 (25.0) | 0 | 1 (25.0) | 0 | 3 (75.0) |
| CIN3 | 7 (28.0) | 9 (36.0) | 9 (36.0) | 0 | 9 (36) | 0 | 0 | 16 (64.0) | 0 | 0 | 8 (32.0) | 17 (68.0) | 11 (44.0) | 7 (28.0) | 7 (28.0) | 0 | 11 (45.8) | 4 (16.7) | 9 (37.5) |
| MicSCC | 2 (22.2) | 3 (33.3) | 4 (44.4) | 0 | 2 (25) | 0 | 1 | 6 (75) | 0 | 0 | 5 (62.5) | 3 (37.5) | 5 (62.5) | 0 | 3 (37.5) | 0 | 7 (77.8) | 2 (22.2) | 0 |
| InvSCC | 18 (75) | 4 (16.7) | 2 (8.3) | 0 | 2 (8.3) | 0 | 3 (12.5) | 19 (79.2) | 0 | 2 (8.3) | 15 (62.5) | 7 (29.2) | 13 (54.2) | 0 | 10 (41.7) | 1 (4.2) | 15 (62.5) | 9 (37.5) | 0 |
|
PRDX2, cytoplasmic expression
|
NCF2, cytoplasmic expression
|
NCF2, sporadic cells
|
NCF2, stroma
|
TPM4, cytoplasmic expressiom
|
|||||||||||||||
| 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ | 3+ | 0 | 1+ | 2+ | 3+ | 0 | 1+ | ||
| SCE | 1 (20.0) | 4 (80.0) | 0 | 0 | 4 (50.0) | 4 (50.0) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 (12.5) | 7 (87.5) | |
| CIN2 | — | — | — | — | 1 (25.0) | 3 (75.0) | 0 | 0 | 3 (75) | 0 | 0 | 1 (25.0) | 3 (75.0) | 0 | 0 | 1 (25.0) | 1 (25.0) | 3 (75.0) | |
| CIN3 | 7 (63.3) | 4 (36.4) | 0 | 0 | 11 (44.0) | 14 (56.0) | 0 | 0 | 11 (84.6) | 0 | 1 (7.7) | 1 (7.7) | 11 (84.6) | 0 | 0 | 2 (15.4) | 7 (28.0) | 18 (62.0) | |
| MicSCC | 0 | 7 (100) | 0 | 0 | 2 (22.2) | 7 (77.8) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 (44.4) | 5 (55.6) | |
| InvSCC | 8 (80.0) | 2 (20.0) | 0 | 0 | 17 (70.8) | 6 (25) | 1 (4.2) | 0 | 9 (37.5) | 0 | 10 (41.7) | 5 (20.8) | 20 (86.9) | 1 (4.3) | 2 (8.6) | 0 | 18 (75) | 6 (25) | |
| (B) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
ANXA6*
|
HSP27*
|
PRDX2*
|
NCF2*
|
TPM4*
|
||||||||||||||||
|
Cytoplasmic
|
Cytoplasm
|
Nuclear
|
Stromal
|
cytoplasmic
|
Cytoplasmic
|
Stromal
|
Cytoplasmic
|
|||||||||||||
| Diagnosis | Diffuse | P-value | Sporadic | P-value | P-value | P-value | P-value | P -value | Diffuse | P -value | Sporadic | P -value | P -value | P -value | ||||||
| SCE | 0 | 0 | 5.0±0 | 1, 3 | 0 | 1.5±2.07 | 1, 3 | 3.2±1.79 | 1 | 1.63±1.77 | 1, 2 | 0 | 0 | 3.38±1.41 | 2 | |||||
| CIN 2 | 2.25±2.63 | 1 | 2.25±1.50 | 1 | 5.75±0.50 | 1, 2 | 2.25±2.63 | 1 | 3.75±2.50 | 1, 2 | — | 2.75±1.89 | 1 | 0.5±1.0 | 1 | 0.75±1.50 | 1 | 3.0±2.0 | 1 | |
| CIN 3 | 3.33±2.03 | 1, 2, 3 | 1.86±1.49 | 1, 2, 3 | 5.67±0.48 | 2, 3, 4 | 2.10±1.97 | 1, 2, 3 | 2.30±2.39 | 2, 3, 4 | 1.50±1.92 | 1, 2 | 1.90±1.92 | 2, 3 | 0 | 0 | 2.52±1.78 | 1, 2, 3 | ||
| MicSCC | 3.56±2.07 | 2, 4 | 2.56±1.67 | 2 | 5.38±0.52 | 1.75±2.43 | 2 | 0.89±1.76 | 4.0±0 | 2, 3 | 3.11±1.76 | 3, 4 | 0 | 0 | 2.22±2.11 | 4 | ||||
| InvSCC | 1.08±1.93 | 3, 4 | 2.71±1.16 | 3 | 5.21±0.53 | 4 | 1.83±2.18 | 3 | 1.5±1.98 | 4 | 0.8±1.69 | 3 | 1.21±1.93 | 4 | 1.46±1.35 | 1 | 0.75±1.73 | 1 | 0.96±1.71 | 3, 4 |
| P-value | 0.358754 | 1 | 0.634305 | 1 | 0.00119 | 1 | 0.892191 | 1 | 0.12707 | 1 | 0.11240458 | 1 | 0.3330842 | 1 | 0.188829 | 1 | 1 | 1 | 0.633935 | 1 |
| 0.786863 | 2 | 0.266001 | 2 | 0.755778 | 2 | 0.695733 | 2 | 0.28235 | 2 | 0.003522 | 2 | 0.7233573 | 2 | 0.23591 | 2 | |||||
| 0.00045 | 3 | 0.0373 | 3 | 0.00064 | 3 | 0.676471 | 3 | 0.41439 | 3 | 0.000168 | 3 | 0.1180145 | 3 | 0.00435 | 3 | |||||
| 0.00305 | 4 | 0.00703 | 4 | 0.23065 | 4 | 0.015041 | 4 | 0.0852 | 4 | |||||||||||
Abbreviations: CIN=cervical intraepithelial neoplasia; InvSCC=invasion SCC; MicSCC=microinvasion SCC; SCC=squamous cervical cancer; SCE=squamous cervical epithelial.
* Histoscore: score of the intensity of expression plus the score of the number of cells that express the protein. Bold values stand for statistically significant differences.