Figure 4.

MΦ in culture promote CXCL12 production. (A) Morphology of adherent layer of Dexter culture 24 h after addition of PBS or clodronate liposomes (10×; bars, 100 µm). (B) Adherent cells were analyzed by flow cytometry for CD115⁺ cells. (A and B) Representative data from two independent experiments are shown. (C) CXCL12 levels were assessed by ELISA at 24 h (left bars) and 72 h (right bars) after liposomal incubation. (D) CXCL12 levels were assessed 3 d after MS-5 cells were co-cultured with (MΦ) or without (Ctrl) BMDM. (E) Levels of CXCL12 secreted from MS-5 cells after culture with medium conditioned by BMDM (MΦ CM) or with control medium (Ctrl). (F) CXCL12 levels were measured after culture of MS-5 cells with control (Ctrl, blue) or MΦ-conditioned (MΦ CM, red) medium that was untreated (left two bars) or treated with Proteinase K (right two bars). (C–F) Representative data from at least two independent experiments are shown.