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. 2011 Feb 14;208(2):227–234. doi: 10.1084/jem.20101459

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© 2011 Bigley et al.

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Figure 4. — Depletion of specific compartments of CD34⁺ BM cells. Flow cytometric analysis of CD34⁺ progenitor compartments in BM according to a recent protocol (Doulatov et al., 2010). Control (performed 7 times); subjects 1, 3, and 4, as indicated (performed twice). Total BM mononuclear cells are shown. The minimum number of CD34⁺ cells analyzed was 4,500. Numbers show the percentage of CD34⁺ cells in each gate. Gate 1 contains CD45⁺ cells; gate 2 contains CD34⁺ cells; gate 3 contains committed CD38⁺ fraction; gate 4 contains a primitive CD38⁻ fraction. All definitions according to Doulatov et al., 2010. HSC, hematopoietic stem cell; MPP, multipotent progenitor; B/NK, committed B/NK precursor; CMP, common myeloid progenitor; MEP megakaryocyte/erythroid progenitor. In this analysis, it was not possible to separate Flt-3⁺ CMP from Flt-3⁻ MEP because Flt3 was not expressed by the subjects’ CD34⁺ cells; thus, CMP and MEP were combined.