Table I.
Transplantation of 0×-, 0–2×-, or ≥5×-divided LKS cell into lethally irradiated mice reveals steady-state cycling heterogeneity in HSC
| Cells injected | Cell dose | Secondary recipient mice with long-term multilineage reconstitution | ||
| Sorting time points after primary transplantation | ||||
| Week 3 | Week 7 | Week 12/14 | ||
| 0×-divided | 1 | 24 (5/21) | 22 (4/18) | 28 (5/18) |
| 0–2×-divided | 10 | 67 (4/6) | 100 (5/5) | ND |
| 0–2×-divided | 20 | 100 (7/7) | ND | ND |
| ≥5×-divided | 10 | 0 (0/4) | 0 (0/5) | 11 (1/9) |
| ≥5×-divided | 20 | 0 (0/7) | 0 (0/7) | 10 (1/10) |
| ≥5×-divided | 50 | 0 (0/7) | 20 (1/5) | 36 (4/11) |
| ≥5×-divided | 250 | ND | 67 (2/3) | 71 (5/7) |
The indicated numbers of LKS cells at the indicated time points after primary transplantation were deposited into individual wells of 96-well plates. The contents of each well were injected into lethally irradiated animals along with 2 × 105 total BM cells. After 4 mo, PB of transplanted mice was analyzed on flow cytometry to identify mice that were multilineage reconstituted by donor cells (above background: >0.1% in B220+, CD3ε+, and B220−CD3ε−CD11b+Gr-1+ cells). The numbers in the time point columns represent the percentage of engrafted mice, and the parenthetical numbers represent the number of engrafted mice per number of transplanted mice. ND, not determined.