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. Author manuscript; available in PMC: 2011 Feb 16.
Published in final edited form as: DNA Repair (Amst). 2005 Jul 12;4(7):793–805. doi: 10.1016/j.dnarep.2005.04.019

Fig. 6.

Fig. 6

Effect of single-stranded U-25-mer DNA on the intrinsic protein fluorescence of UNG and Arg276 mutant proteins. Each Arg276 mutant protein (100 nM) was mixed with ssU-25-mer DNA (1 μM) in buffer A at 25 °C and the intrinsic protein fluorescence monitored using a stopped-flow spectrometer. Each kinetic trace shown represents an average of 10 individual acquisitions; the identity of the wild-type UNG and Arg276 mutant proteins is indicated in the lower right corner of the time trace. The solid lines represent the best-fit curves to Eq. (2) as described in Section 2.