Table 1. Oligonucleotide sequences and their alignment with the duplex target.
| Target | 5′ T C T C C A C C C A C C T C T C G C A C T C T C C C T T C T C C T T T 3′ | Kd (µM) |
| |
3′ A
G A G G T G G G T G G A G A G C G T G A G A G G G A A G A G G A A A 5′ |
|
| TFO-p | 3′ G A G A G G G A A G A G G A A A -5′ | >50 |
| TFO1 | 5′ G A G A G G G A A G A G G A A A -3′ | 0.8 |
| TFO2 | 5′ G A G A G G G A A G A G G 3′ | 0.2 |
| TFO3 | 5′ G G A G A G C G T G A G A G G G A A G A G G 3′ | >50 |
| TFO4 | 5′ G G A G A G C G A G A G A G G G A A G A G G 3′ | >50 |
| TFO5 | 5′ G G A G A G C G G G A G A G G G A A G A G G 3′ | >50 |
| TFO6 | 5′ G G A G A G G G T G A G A G G G A A G A G G 3′ | >50 |
| TFO7 | 5′ G G A G A G G G A G A G A G G G A A G A G G 3′ | >50 |
| TFO8 | 5′ G G A G A G G G G G A G A G G G A A G A G G 3′ | >50 |
| TFO9 | 5′ G G A G A G A G T G A G A G G G A A G A G G 3′ | >50 |
| TFO10 | 5′ G G A G A G A G A G A G A G G G A A G A G G 3′ | >50 |
| TFO11 | 5′ G G A G A G A G G G A G A G G G A A G A G G 3′ | >50 |
| TFO12 | 5′ G G A G A G T G T G A G A G G G A A G A G G 3′ | 6 |
| TFO13 | 5′ G G A G A G T G A G A G A G G G A A G A G G 3′ | 1.5 |
| TFO14 | 5′ G G A G A G T G G G A G A G G G A A G A G G 3′ | 0.6 |
| TFO15 | 5′ A G A G G A G G G A G G A G A G T G A G A G A G G G A A G A G G 3′ | 0.2 |
| TFO16 | 5′ A G A G G A G G G A G G A G A G T G G G A G A G G G A A G A G G 3′ | 0.1 |
| TFO17 | 5′ G G A G A G T G A G A G A G G G A A G A G G A A A 3′ | 5 |
| TFO18 | 5′ G G A G A G T G G G A G A G G G A A G A G G A A A 3′ | 2 |
| TFO19 | 5′ A G A G G A G G G A G G A G A G T G A G A G A G G G A A G A G G A A A 3′ | 0.6 |
| TFO20 | 5′ A G A G G A G G G A G G A G A G T G G G A G A G G G A A G A G G A A A 3′ | 0.3 |
The equilibrium dissociation constant (Kd) for third strand binding was estimated as the concentration of TFO at which apparent binding was half maximal, as judged by gel mobility shift assay in Figure 2.