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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Biomed Microdevices. 2011 Feb;13(1):69–87. doi: 10.1007/s10544-010-9472-8

Table 1.

Primary and secondary antibodies. For VE-cadherin and KLF2, cells were permeabilized with 0.1% Triton-X 100 (Sigma) for 4 min. All cells were blocked with 10% horse serum (Vector, Burlington, ON) for 30 min and probed with antibodies diluted in 2% BSA (Sigma) for 30 min

Primary antibody, 1:50 dilution Secondary antibody, 1:100 dilution
Anti-human VCAM-1, goat serum IgG (R&D Systems, Minneapolis, MN) Alexa Fluor 488 donkey anti-goat IgG (Iinvitrogen, Burlington, ON)
Anti-human ICAM-1, mouse monoclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) Alexa Fluor 568 rabbit anti-mouse IgG (Invitrogen)
Anti-human VE-cadherin, rabbit IgG (Sigma, Oakville, ON) Alexa Fluor 488 donkey anti-rabbit (Invitrogen)
Anti-human KLF2 (lung KLF), goat polyclonal IgG (Santa Cruz Biotechnology). Alexa Fluor 546 donkey anti-goat (Invitrogen)