Table 1.
Primary antibody, 1:50 dilution | Secondary antibody, 1:100 dilution |
---|---|
Anti-human VCAM-1, goat serum IgG (R&D Systems, Minneapolis, MN) | Alexa Fluor™ 488 donkey anti-goat IgG (Iinvitrogen, Burlington, ON) |
Anti-human ICAM-1, mouse monoclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) | Alexa Fluor™ 568 rabbit anti-mouse IgG (Invitrogen) |
Anti-human VE-cadherin, rabbit IgG (Sigma, Oakville, ON) | Alexa Fluor™ 488 donkey anti-rabbit (Invitrogen) |
Anti-human KLF2 (lung KLF), goat polyclonal IgG (Santa Cruz Biotechnology). | Alexa Fluor™ 546 donkey anti-goat (Invitrogen) |