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. 2010 Dec 23;12(1):1–11. doi: 10.3390/ijms12010001

Figure 4.

Figure 4

Binding behaviors of scFv (H4) (♦) and the scFv (H44-L100) (■) mutant in non-competitive ELISA (a) and competitive ELISA (b). (a) Wells were coated with 25 μL, 50 μM AFB1-BSA; (b) 2.5 μM scFv was preincubated with free AFB1 (0.02–50 ng/mL) at 37 °C for 1 h.