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A 1.1% agarose gel electrophoresis of ds cDNA after PCR. One μg (3 μL) of Poly(A)+ RNA was used as RNA template with the indicated primers in a first-strand synthesis. 11 μL of ss cDNA served as a template for primer-extension based, second-strand synthesis using 3 thermal cycles. Lane M: HyperLadder marker (5 μL). Lane 1: 5 μL sample of the ds cDNA product showing a smear ranging from 0.2 to 3 kb.
