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. 2011 Jan 18;12(1):613–626. doi: 10.3390/ijms12010613

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© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) The titer of unamplified library. (B) Blue and white screening of the unamplified library. Arrows indicate blue plaques. The two plates (A, B) were inoculated with a 10-fold dilution of packaged phage. Plaques were approximately 1 mm in diameter. (C) The titer of amplified library. (D) Blue and white screening of the amplified library. Arrows indicate blue plaques. The two plates (C, D) were inoculated with a 1 × 10⁴ dilution of phage lysate. Bacteriophage: λ lcI857 Sam7. Host: Escherichia coli LE392MP.

(A) The titer of unamplified library. (B) Blue and white screening of the unamplified library. Arrows indicate blue plaques. The two plates (A, B) were inoculated with a 10-fold dilution of packaged phage. Plaques were approximately 1 mm in diameter. (C) The titer of amplified library. (D) Blue and white screening of the amplified library. Arrows indicate blue plaques. The two plates (C, D) were inoculated with a 1 × 10⁴ dilution of phage lysate. Bacteriophage: λ lcI857 Sam7. Host: Escherichia coli LE392MP.