Table 4.
List of the three most common matrix types—2,5-dihydroxybenzoic acid (DHB), α-cyano-4-hydroxycinnamic acid (CHCA) and 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid, SA) as well as their documented modifications - for MALDI mass spectrometry. Suitability for measurement of bio-molecules is specified [26].
| Matrix | Chemical name | Biomolecule specificity |
|---|---|---|
| DHB | 2,5-dihydroxybenzoic acid | Lipids, peptides, <10 kDa proteins |
| DHB/aniline | DHB + aniline | Lipids, peptides, <10 kDa proteins |
| DHB/3-AP | DHB + 3-acetyl pyridine | Lipids, peptides, <10 kDa proteins |
| CHCA | α-cyano-4-hydroxycinnamic acid | Peptides, small proteins (<10 kDa) |
| CHCA/aniline | CHCA + aniline | Peptides, <10 kDa proteins |
| SA | 3,5-dimethoxy-4-hydroxycinnamic acid | Proteins (>10 kDa) |
| SA/aniline | SA + aniline | Proteins (>10 kDa) |
| SA/3-AP | SA + 3-acetyl pyridine | Proteins (>10 kDa) |
| SA/HFIP | SA + 1,1,1,3,3,3-hexafluoro-2-propanol | Proteins (>30 kDa) |
| SA/TFE | SA + 2,2,2-trifluoroethanol | Proteins (>30 kDa) |