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. 2001 Feb 1;29(3):589–597. doi: 10.1093/nar/29.3.589

Figure 2.

Figure 2

Ubiquitous expression of PCAT1–4 mRNAs. (A) Northern blot using total RNA isolated from A.thaliana seedling, stem, rosette or flower tissue. The blot was probed with a PCAT2 fragment and shows two hybridizing bands of ~5.5 and 7.3 kb. Both mRNAs are of sufficient length to harbor the complete PCAT2 protein coding region, which is 4.7 kb in length. The 5.5 kb band is marked by an arrow because it is likely to represent the mature form of the PCAT2 transcript. The panel at the bottom shows the hybridization signal of the 25S ribosomal RNA serving as a loading control. (B) PCAT1, 2, 3 and 4 transcripts are expressed in early stages of development (seedlings) and in the three major tissues (rosette, stem and flowers) of the adult plant. Fragments of each of the four PCAT transcripts (see Table 1 for location of amplified fragments) were amplified by RT–PCR from total RNA isolated from the indicated tissue. Amplification was stopped while it was still in the linear range. Signals should not be taken to reflect the precise relative or absolute levels of mRNA abundance.