Table 1.

Strategy for the generation of the eight genotypes used in this publication, litter size, and Mendelian genotype ratio

Mating		Litter size Pups/litter (n)	Mendelian ratio
Mother	Father		Offspring	Expected (%)	Observed (%)
Mct8+/−	Mct8+/y	8.07 ± 0.45	Male Mct8+/y (Wt)a	2550	23.14
			Male Mct8−/y (M8KO)a	25	23.14
			Female Mct8+/+ and Mct8+/−	50	53.72
Mct8+/−D1−/−	Mct8+/yD1−/−	7.67 ± 0.35	Male Mct8+/yD1−/− (D1KO)a	25	29.56
			Male Mct8−/yD1−/− (M8D1KO)a	25	25.22
			Female Mct8+/+D1−/− and Mct8+/−D1−/−	50	45.22
Mct8+/−D2−/−	Mct8+/yD2−/−	8.67 ± 0.42	Male Mct8+/yD2−/− (D2KO)a	25	28.46
			Male Mct8−/yD2−/− (M8D2KO)a	25	23.08
			Female Mct8+/+D2−/− and Mct8+/−D2−/−	50	48.46
Mct8+/−D1−/−D2−/−	Mct8+/yD1−/−D2−/−	7.67 ± 0.57	Male Mct8+/yD1−/−D2−/− (D1D2KO)a	25	20.00
			Male Mct8−/yD1−/−D2−/− (M8D1D2KO)a	25	25.22
			Female Mct8+/+D1−/−D2−/− and Mct8+/−D1−/−D2−/−	50	54.78

Fifteen litters were tested in each mating. The litter size is expressed as mean ± se.

^aGenotypes used in these studies.