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. 2011 Jan 14;152(3):890–902. doi: 10.1210/en.2010-0716

Fig. 3.

Fig. 3.

Response to exogenous NAPE administration in MC4R−/− and SIM-1+/− mice. A, NAPE decreased food intake in age- and weight-matched female mice as compared with vehicle groups on normal chow (***, P < 0.001 vs. NAPE injected same genotype group; *, P < 0.05 vs. vehicle injected WT, t test, WT = 7, MC4R+/− = 7, MC4R−/− = 10). B, Percent reduction of food intake from data in A. At 16 h, MC4R−/− mice showed greater cumulative reduction of food intake after 100 mg/kg NAPE injection compared with WT and MC4R+/− mice (***, P < 0.001, one-way ANOVA). C, 100 mg/kg NAPE ip injection reduced food intake in high-fat diet–fed 4-month-old female mice (***, P < 0.001; *, P < 0.05 vs. NAPE injected group, t test WT = 7, MC4R−/− = 8). D, Cumulative food intake at 16 h after injection from C. High-fat diet–fed MC4R−/− mice showed hypersensitivity to NAPE at 16 h after injection (***, P < 0.001; *, P < 0.05, t test). E, Age- and weight-matched male SIM-1+/− mice showed greater reduction of cumulative food intake at 21 h after 250 mg/kg NAPE injection compared with vehicle-injected group (***, P < 0.001, t test, WT vehicle = 6, WT NAPE = 7, SIM-1+/− vehicle = 5, SIM-1+/− NAPE = 5). All data are presented as mean ± sem.