Table 3.

Plasma NAE level (μM) of free feeding mice maintained on low-fat diet and high-fat diet

	WT-LF	WT-HF	KO-LF	KO-HF
C22_5	0.1609 ± 0.0155	0.1595 ± 0.0145	0.1700 ± 0.0096	0.1792 ± 0.0088
C22_6	0.0282 ± 0.0051	0.0209 ± 0.0018	0.0178 ± 0.0019*	0.0143 ± 0.0012*
C20_2	0.0152 ± 0.0004	0.0185 ± 0.0001#	0.0084 ± 0.0008*	0.0118 ± 0.0010*#
C20_3	0.0117 ± 0.0008	0.0159 ± 0.0005#	0.0069 ± 0.0009*	0.0103 ± 0.0009*#
C20_4	0.0237 ± 0.0029	0.0294 ± 0.0017	0.0144 ± 0.0026	0.0199 ± 0.0019*
C20_5	0.0181 ± 0.0020	0.0186 ± 0.0007	0.0101 ± 0.0014*	0.0125 ± 0.0009*
C18_0	0.1149 ± 0.0084	0.1354 ± 0.0094	0.0616 ± 0.0064*	0.0863 ± 0.0079*#
C18_1	0.1632 ± 0.0089	0.1741 ± 0.0133	0.0878 ± 0.0076*	0.1107 ± 0.0089*
C18_2	0.1174 ± 0.0038	0.1098 ± 0.0062	0.0601 ± 0.0065*	0.0700 ± 0.0062*
C18_3	0.0502 ± 0.0016	0.0441 ± 0.0020	0.0248 ± 0.0026*	0.0265 ± 0.0025*
C16_0	0.1351 ± 0.0093	0.1292 ± 0.0046	0.0802 ± 0.0091*	0.0861 ± 0.0065*
C16_1	0.1034 ± 0.0037	0.0937 ± 0.0046	0.0619 ± 0.0065*	0.0622 ± 0.0049*
Total	0.9422 ± 0.0344	0.9493 ± 0.0403	0.6046 ± 0.0417*	0.6902 ± 0.0427*

NAEs were determined by LC/MS/MS. Values are expressed as mean ± sem.

^*, P < 0.05 compared with WT maintained on the same diet;

^#, P < 0.05 compared with the same genotype maintained on different diet, one-way ANOVA, WT = 5, MC4R−/− = 7.