Figure 1.

Glucose-repressive gene products on 2D gels induced in B.subtilis cells during growth in DSM. Total proteins from strain 1A250 (wild-type) (A) and strain 1A147 (ccpA1) (B) grown in DSM to an A₆₀₀ of 1.0 with and without 10 mM glucose (Glc) were subjected to 2D gel electrophoresis as described in the text. Identification of the proteins in 11 glucose-sensitive spots, which are indicated by red arrowheads and numbers, is shown in Table 1. The 2D gel patterns (B) for strain 1A147 cells indicated that out of the 11 spots, four were still glucose-sensitive (indicated by red arrowheads), whereas the others (green arrowheads) had become glucose-insensitive due to the ccpA1 mutation.