Table 1. Identification of the B.subtilis glucose-repressive gene products on 2D gels^a.

Spot no.	Sequence	Protein	Protein descriptionb	CcpA dependencyc
1	MKLXFNEATTLE	IolI	Inositol utilization (unspecified)	–
2	MDKTISVIGMPM	RocF	Arginase	+
3	MNIHEYQGKEVL	SucC	Succinyl CoA synthetase β chain	+
4	TALSKSKEIIDQ	RocD	Ornithine aminotransferase	+
5	MRMVDIIIKKQN	Pdp	Pyrimidine nucleoside phosphorylase	+
6	LVKEAGKPXKEA	RocA	Pyrroline-5-carboxylate dehydrogenase	+
7	AEIRKLKNYING	IolA	Oxidative decarboxylase in inositol catabolism	–
8	MNSVDLTADLXA	PckA	Phosphoenolpyruvate carboxykinase	–
9	MKKAKLGKSDLQ	IolS	Inositol catabolism (unspecified)	–
10	SVFINKDTRVIV	SucD	Succinyl CoA synthetase α chain	+
11	TATIIDGKEXAR	FolD	Methylenetetrahydrofolate dehydrogenase	+

^aTotal protein from strain 1A250 (wild-type) cells grown to A₆₀₀ = 1.0 in DSM without glucose was subjected to preparative 2D gel electrophoresis, as described in the text. The proteins in the spots corresponding to those shown in the left panel of Figure 1A were extracted and sequenced. N-terminal sequencing of the proteins in the spots allowed us to identify the B.subtilis genes encoding these products by means of the SubtiList worldwide web server (http://www.pasteur.fr/Bio/SubtiList) with the FASTA program (48).

^bThe protein descriptions were mainly obtained from the SubtiList World Wide Web server.

^cThe CcpA dependency of the glucose repression of the synthesis of each protein was obtained from the results (Fig. 1B).