Table 2. The glucose-repressive genes detected through microarray analysis.
| Genea |
Functiona |
Ratio (wt)b |
Ratio (ccpA1)b |
| glmS | l-glutamine-d-fructose-6-phosphate amidotransferase | 7.2 | 3.5 |
| ybcM | similar to d-glucosamine-d-fructose-6-phosphate aminotransferase | 5.4 | <2 |
| glpQ | glycerophosphoryl diester phosphodiesterase | 5.5 | <2 |
| ycnKc | similar to transcriptional regulator (DeoR family) | 6.4 | <2 |
| yerA | similar to adenine deaminase | >100 | <2 |
| yesL | similar to unknown proteins from B.subtilis | 14.0 | <2 |
| yesM | similar to two-component sensor histidine kinase | 8.2 | <2 |
| yfmQ | unknown function | 12.5 | <2 |
| acoAc,d | acetoin dehydrogenase E1 component (TPP-dependent α subunit) | >100 | 12.9 |
| acoCc | acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase) | 58.0 | 5.3 |
| glpF | glycerol uptake facilitator | 5.3 | <2 |
| yisS | similar to myo-inositol 2-dehydrogenase | 20.6 | 4.7 |
| ykuL | unknown function | 6.7 | <2 |
| ctaC | cytochrome caa3 oxidase (subunit II) | 31.3 | <2 |
| ctaDd | cytochrome caa3 oxidase (subunit I) | 37.3 | <2 |
| ctaE | cytochrome caa3 oxidase (subunit I) | 22.6 | <2 |
| ctaG | function unknown | 7.8 | <2 |
| sucC | succinyl-CoA synthetase (β subunit) | 5.2 | <2 |
| sucD | succinyl-CoA synthetase (α subunit) | 5.1 | <2 |
| tdh | threonine 3-dehydrogenase | 10.6 | <2 |
| yocH | similar to cell wall-binding protein | 6.3 | <2 |
| qcrB | menaquinol:cytochrome c oxidoreductase (cytochrome b subunit) | 5.7 | <2 |
| qcrA | menaquinol:cytochrome c oxidoreductase (iron-sulfur subunit) | 16.0 | <2 |
| resE | two-component sensor histidine kinase involved in aerobic and anaerobic respiration | 6.7 | <2 |
| sacC | levanase | 14.4 | <2 |
| cstAd | carbon starvation-induced protein | 6.2 | <2 |
| araA | l-arabinose isomerase | 7.5 | <2 |
| speD (ytcF) | unknown function | 13.2 | 2.0 |
| gapB | glyceraldehyde-3-phosphate dehydrogenase | >100 | 25.2 |
| pckA | phosphoenolpyruvate carboxykinase | 26.8 | 3.7 |
| ytkA | unknown function | 6.1 | <2 |
| dhbC | isochorismate synthase | 5.5 | <2 |
| yvfL | similar to maltodextrin transport system permease | 5.6 | <2 |
| yvfK | similar to maltose/maltodextrin-binding protein | 8.3 | <2 |
| yvdR | similar to molecular chaperone | 26.4 | 7.1 |
| gerBC | germination response to the combination of glucose, fructose, l-asparagine and KCl | 23.0 | <2 |
| ywtD | similar to murein hydrolase | 5.8 | <2 |
| rbsRd | transcriptional repressor of the ribose operon | >100 | <2 |
| rbsK | ribokinase | >100 | <2 |
| rbsD | ribose ABC transporter (membrane protein) | >100 | <2 |
| rbsAd | ribose ABC transporter (ATP-binding protein) | >100 | <2 |
| rbsB | ribose ABC transporter (ribose-binding protein) | >100 | <2 |
| ywsB | similar to unknown proteins from B.subtilis | 32.2 | <2 |
| ywsA | unknown function | 23.2 | <2 |
| ywqM | similar to transcriptional regulator (LysR family) | 16.0 | <2 |
| rocA | pyrroline-5 carboxylate dehydrogenase | 5.6 | <2 |
| rocG (yweB) | glutamate dehydrogenase (major) | 48.6 | <2 |
| licA | PTS lichenan-specific enzyme IIA component | 5.2 | <2 |
| msmXc,d | similar to multiple sugar-binding transport ATP-binding protein | 9.2 | <2 |
| yxeF | unknown function | >100 | 22.3 |
| iolJc | aldolase in myo-inositol catabolism | 28.4 | 4.5 |
| iolIc | unknown function in myo-inositol catabolism | >100 | 9.2 |
| iolHc | unknown function in myo-inositol catabolism | >100 | 22.3 |
| iolGc | myo-inositol 2-dehydrogenase | >100 | 9.2 |
| iolFc | myo-inositol permease | >100 | 6.5 |
| iolEc | 2-inosose dehydratase | >100 | 8.3 |
| iolDc | 2,3-diketo-4-deoxy-epi-inositol hydrolase | >100 | 14.4 |
| iolCc | kinase in myo-inositol catabolism | >100 | 7.0 |
| iolBc,d | unknown function in myo-inositol catabolism | 91.5 | 7.5 |
| iolAc | oxidative decarboxylase in myo-inositol catabolism | 87.8 | 6.0 |
| iolR | transcriptional repressor of the inositol operon | 6.7 | 4.2 |
| iolS | unknown function in myo-inositol catabolism | 5.0 | 2.0 |
| yxbF | unknown function | 15.3 | 3.6 |
| rocF | arginase | 5.5 | <2 |
| rocE | amino acid permease | 7.5 | <2 |
| rocD | ornithine aminotransferase | 8.3 | <2 |
aThe gene names and functions are basically as in the literature (13) and SubtiList (http://genolist.pasteur.fr/SubtiList). The functions of some iol genes were deduced from unpublished results (K.Yoshida and Y.Fujita).
bThe repression ratios [wild-type (wt) and ccpA1] were calculated by dividing the spot intensities obtained for cells grown without glucose by those with glucose after subtraction of their backgrounds and subsequent normalization.
cGlucose repression of the expression of these genes were confirmed on first level analysis of pMUTIN integrants, the results of which are obtainable from JAFAN (http://bacillus.genome.ad.jp).
dPutative catabolite responsible elements (cres) are associated with these genes (9).