Table 3. Glucose repression of the synthesis of inositol dehydrogenase encoded by iolG.

Strain	Relevant genotype	Glucose repression ratioa (fold, +Iol/+Iol and Glc)
1A250	Wild-type	>350
1A147	ccpA1	4.9
FU706	iolR::neo	6.1
FU707	ccpA1 iolR::neo	1.1

^aCells of B.subtilis strains were grown to an A₆₀₀ of 0.8 in S6 medium (25) containing 0.5% casamino acids with 10 mM inositol (Iol), or 10 mM each of inositol and glucose (Glc). The cells (4.5 A₆₀₀ units) were harvested and then treated with lysozyme and sonicated briefly to prepare crude extracts (39). The inositol dehydrogenase activity in the crude extracts was measured as described previously (10). The repression ratios, folds, were obtained by dividing the enzyme activities in the crude extracts prepared from cells grown with glucose by those in the case of growth with inositol and glucose.