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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Methods Mol Biol. 2011;686:357–369. doi: 10.1007/978-1-60761-938-3_17

Figure E.

Figure E

Confocal microscopy of the physiologic barrier to drug transport. A cross sectional confocal image of a Dm brain at the lobular plate hemolymph injected with 10 kDa tetramethyl-rhodamine dextran (TMRD). Both brain are marked with a pan glial driver (REPO-GAL4) crossed to a transgene expressing GFP (green channel) using the UAS/GAL4 system of Brand and Perrimon (23). On left the moody mutant shows strong dextran signal (red) penetrating the brain. On the right is the moody mutant rescued with Moody-GFP showing wild type BBB function. Here the dextran is fixed to surface tissue outside the brain (red).