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GRP78 and CPS1 were validated by Western blot analysis. GAPDH was used as loading control. A three dimensional view (3D view) of the spots corresponding to the selected proteins is displayed. 20 μg of liver lysate of each sample was incubated against each antibody in order to validate the differences in expression observed by DIGE. GRP78 and CPS1 were down-regulated in NASH samples.
